In Press
Ting Hai MD, Yannis Amador MD, Feroze Mahmood MD, Jelliffe Jeganathan MBBS, Arash Khamooshian MD, Ziyad O Knio BS, Robina Matyal MD, Alina Nicoara MD, David C Liu MD, Venkatachalam Senthilnathan MD, et al. Changes in Tricuspid Annular Geometry in Patients with Functional Tricuspid Regurgitation. Journal of Cardiothoracic and Vascular Anesthesia. In Press.
Rabia Amir MD, Ziyad Knio BS, Feroze Mahmood MD, Achikam Oren-Grinberg MD, Akiva Leibowitz MD, Ruma Bose MD, Shahzad Shaefi MD, John Mitchell MD, Muneed Ahmed MD, Amit Bardia MD, et al. Ultrasound as a Screening Tool for Central Venous Catheter Positioning and Exclusion of Pneumothorax. Critical Care Medicine. 2017.
Jelliffe Jeganathan MBBS, Rabya Saraf BA, Feroze Mahmood MD, Anam Pal MD, Manoj Bhasin PD, Thomas Huang BS, Aaron Mittel MD, Ziyad Knio BS, Russell Simons BS, Kamal Khabbaz MD, et al. Mitochondrial dysfunction in atrial tissue of patients developing post-operative atrial fibrillation. Annals of Thoracic Surgery. 2017.
Jelliffe Jeganathan MBBS, Ziyad Knio BS, Yannis Amador MD, Ting Hai MD, Arash Khamooshian MD, Robina Matyal MD, Kamal Khabbaz MD, Feroze Mahmood MD. Artificial intelligence in mitral valve analysis. Annals of Cardiac Anaesthesia. 2017;20 (2) :129-134.
Derek Eshun MD, Rabya Saraf BA, Soochan Bae MD, Jelliffe Jeganathan MBBS, Feroze Mahmood MD, Serkan Dilmen MD, Qingen Ke MD, Dongwon Lee PD, Peter Kang MD, Robina Matyal MD. Neuropeptide Y3-36 Incorporated Into PVAX Nanoparticle Improves Functional Blood Flow In A Murine Model Of Hind Limb Ischemia. Journal of Applied Physiology [Internet]. 2017. Publisher's Version
Feroze Mahmood MD, BS ZK, Lu Yeh MD, Rabia Amir MD, Robina Matyal MD, Azad Mashari MD, Robert Gorman MD, Joseph Gorman III MD, Kamal R. Khabbaz MD. Regional Heterogeneity in the Mitral Valve Apparatus in Patients With Ischemic Mitral Regurgitation. Annals of Thoracic Surgery. 2017;103 (4) :1171-1177.
Jelliffe Jeganathan MBBS, Baribeau Y, Bortman J, Feroze Mahmood MD, Marc Shnider MD, Muneeb Ahmed MD, Azad Mashari MD, Rabia Amir MD, Yannis Amador MD, Robina Matyal MD. Use of 3-Dimensional Printing to Create Patient-Specific Thoracic Spine Models as Task Trainers. Regional Anesthesia and Pain Medicine. 2017.
Amit Bardia MBBS, Rabya Saraf BA, Andrew Maslow MD, Kamal Khabbaz MD, Feroze Mahmood MD. The Coanda Effect. Anesthesia & Analgesia. 2016;123 (3) :582-584.
Lars Stangenberg MDPD, Thomas Curran MDPD, Fahad Shuja MD, Robert Rosenberg MD, Feroze Mahmood MD, Marc Schermerhorn MD. Development of a risk prediction model for transfusion in carotid endarterectomy and demonstration of cost-saving potential by avoidance of "type and screen". Journal fo Vascular Surgery. 2016;64 (6) :1711-1718.Abstract

Objectives: Preoperative testing for carotid endarterectomy (CEA) often includes blood 3 typing and antibody screen (T&S). In our institutional experience, however, transfusion 4 for CEA is rare. We assessed transfusion rate and risk factors in a national clinical 5 database to identify a cohort of patients in whom T&S can safely be avoided with the 6 potential for substantial cost savings. 7 Methods: Using the NSQIP database, transfusion events and timing were established for 8 all elective CEAs in 2012-13. Comorbidities and other characteristics were compared for 9 patients receiving intra- or postoperative transfusion and those that did not. After random 10 assignment of the total data to either a training or validation set, a prediction model for 11 transfusion risk was created and subsequently validated. 12 Results: Of 16,043 patients undergoing CEA in 2012-13, 276 received at least one 13 transfusion prior to discharge (1.7%). 42% of transfusions occurred on the day of surgery. 14 Preoperative hematocrit < 30% (Odds ration OR: 57.4; 95% confidence interval CI: 29.6- 15 111.1), history of congestive heart failure (OR: 2.8; 95%CI: 1.1 – 7.1), dependent 16 functional status (OR: 2.7; 95%CI: 1.5-5.1), coagulopathy (OR: 2.5; 95%CI: 1.7-3.6), 17 creatinine ≥ 1.2 mg/dl (OR: 2.3; 95%CI: 1.6-3.3) preoperative dyspnea (OR: 2.0; 95%CI: 18 1.4-3.1) and female gender (OR: 1.6; 95%CI: 1.1-2.3) predicted transfusion. A risk 19 prediction model based on these data produced a C-statistic of 0.85; application of this 20 model to the validation set demonstrated a C-statistic of 0.81. 93% of patients in the 21 validation set received a score of 6 or less corresponding to an individual predicted 22 transfusion risk of 5% or less. Omitting a T&S in these patients would generate a 23 substantial annual cost saving for NSQIP hospitals.

Mario Montealegre-Gallegos MD, Robina Matyal MD, Kamal Khabbaz MD, Khurram Owais MD, Andrew Maslow MD, Philip Hess, MD MPH, Feroze Mahmood MD. Heterogeneity in the Structure of the Left Ventricular Outflow Tract: A 3-Dimensional Transesophageal Echocardiographic Study. Anesthesia & Analgesia. 2016;123 (2) :290-6.
Mario Montealegre-Gallegos MD, Feroze Mahmood MD, Han Kim MD, Remco Bergman MD, John D Mitchell MD, Ruma Bose MD, Katie Hawthorne MD, T. David O'Halloran MD, Vanessa Wong BS, Philip Hess, MD MPH, et al. Imaging skills for transthoracic echocardiography in cardiology fellows: The value of motion metrics. Annals of Cardiac Anesthesia. 2016;19 (2) :245-250.
Khurram Owais MD, Mario Montealegre-Gallegos MD, Jelliffe Jeganathan MBBS, Robina Matyal MD, Kamal Khabbaz MD, Feroze Mahmood MD. Dynamic changes in the ischemic mitral annulus: Implications for ring sizing. Annals of Cardiac Anesthesia. 2016;19 (1) :15-9.Abstract


Contrary to the rest of the mitral annulus, inter-trigonal distance is known to be relatively less dynamic during the cardiac cycle. Therefore, intertrigonal distance is considered a suitable benchmark for annuloplasty ring sizing during mitral valve (MV) surgery. The entire mitral annulus dilates and flattens in patients with ischemic mitral regurgitation (IMR). It is assumed that the fibrous trigone of the heart and the intertrigonal distance does not dilate. In this study, we sought to demonstrate the changes in mitral annular geometry in patients with IMR and specifically analyze the changes in intertrigonal distance during the cardiac cycle.


Intraoperative three-dimensional transesophageal echocardiographic data obtained from 26 patients with normal MVs undergoing nonvalvular cardiac surgery and 36 patients with IMR undergoing valve repair were dynamically analyzed using Philips Qlab ® software.


Overall, regurgitant valves were larger in area and less dynamic than normal valves. Both normal and regurgitant groups displayed a significant change in annular area (AA) during the cardiac cycle (P < 0.01 and P < 0.05, respectively). Anteroposterior and anterolateral-posteromedial diameters and inter-trigonal distance increased through systole (P < 0.05 for all) in accordance with the AAs in both groups. However, inter-trigonal distance showed the least percentage change across the cardiac cycle and its reduced dynamism was validated in both cohorts (P > 0.05).


Annular dimensions in regurgitant valves are dynamic and can be measured feasibly and accurately using echocardiography. The echocardiographically identified inter-trigonal distance does not change significantly during the cardiac cycle.

Azad Mashari MD, Ziyad Knio BS, Jelliffe Jeganathan MBBS, Mario Montealegre-Gallegos MD, Lu Yeh MD, Yannis Amador MD, Robina Matyal MD, Rabya Saraf BA, Kamal Khabbaz MD, Feroze Mahmood MD. Hemodynamic Testing of Patient-Specific Mitral Valves Using a Pulse Duplicator: A Clinical Application of Three-Dimensional Printing. Journal of Cardiothoracic & Vascular Anesthesia. 2016;30 (5) :1278-85.Abstract

To evaluate the feasibility of obtaining hemodynamic metrics of echocardiographically derived 3-dimensional (3D) printed mitral valve models deployed in a pulse duplicator chamber.

Rabya Saraf BA, Feroze Mahmood MD, Rabia Amir MD, Robina Matyal MD. Neuropeptide Y is an Angiogenic Factor in Cardiovascular Regeneration. European Journal of Pharmacology. 2016.Abstract

In diabetic cardiomyopathy, there is altered angiogenic signaling and increased oxidative stress. As a result, anti-angiogenic and pro-inflammatory pathways are activated. These disrupt cellular metabolism and cause fibrosis and apoptosis, leading to pathological remodeling. The autonomic nervous system and neurotransmitters play an important role in angiogenesis. Therapies that promote angiogenesis may be able to relieve the pathology in these disease states. Neuropeptide Y (NPY) is the most abundantly produced and expressed neuropeptide in the central and peripheral nervous systems in mammals and plays an important role in promoting angiogenesis and cardiomyocyte remodeling. It produces effects through G-protein-coupled Y receptors that are widely distributed and also present on the myocardium. Some of these receptors are also involved in diseased states of the heart. NPY has been implicated as a potent growth factor, causing cell proliferation in multiple systems while the NPY3-36 fragment is selective in stimulating angiogenesis and cardiomyocyte remodeling. Current research is focusing on developing a drug delivery mechanism for NPY to prolong therapy without having significant systemic consequences. This could be a promising innovation in the treatment of diabetic cardiomyopathy and ischemic heart disease.

R D, Montealegre-Gallegos M, Matyal R, Belani K, Chawla N. Training the Anesthesiologist in Point-of-Care Ultrasound. International Anesthesiology Clinics. 2016;54 (1) :71-93.
Saraf R, Huang T, Mahmood F, Owais K, Bardia A, Khabbaz K, Liu D, Senthilanathan V, Lassaletta AD, Sellke F, et al. Early Cellular Changes in the Ascending Aorta and Myocardium in a Swine Model of Metabolic Syndrome. PLOS One. 2016;11 (1).Abstract

Background: Metabolic syndrome is associated with pathological remodeling of the heart and adjacent vessels. The early biochemical and cellular changes underlying the vascular damage are not fully understood. In this study, we sought to establish the nature, extent, and initial timeline of cytochemical derangements underlying reduced ventriculo-arterial compliance in a swine model of metabolic syndrome.

Methods: Yorkshire swine (n=16) were fed a normal diet (ND) or a high-cholesterol (HCD) for 12 weeks. Myocardial function and blood flow was assessed before harvesting the heart. Immuno-blotting and immuno-histochemical staining were used to assess the cellular changes in the myocardium, ascending aorta and left anterior descending artery (LAD).

Results: There was significant increase in body mass index, blood glucose and mean arterial pressures (p=0.002, p=0.001 and p=0.024 respectively) in HCD group. At the cellular level there was significant increase in anti-apoptotic factors p-Akt (p=0.007 and p=0.002) and Bcl-xL (p=0.05 and p=0.01) in the HCD aorta and myocardium, respectively. Pro-fibrotic markers TGF-β (p=0.01), pSmad1/5 (p=0.03) and MMP-9 (p=0.005) were significantly increased in the HCD aorta. The levels of pro-apoptotic p38MAPK, Apaf-1 and cleaved Caspase3 were significantly increased in aorta of HCD (p= 0.03, p=0.04 and p=0.007 respectively). Similar changes in coronary arteries were not observed in either group. Functionally, the high cholesterol diet resulted in significant increase in ventricular end systolic pressure and -dp/dt (p=0.05 and p=0.007 respectively) in the HCD group.

Conclusion: Preclinical metabolic syndrome initiates pro-apoptosis and pro-fibrosis pathways in the heart and ascending aorta, while sparing coronary arteries at this early stage of dietary modification.

Rabya Saraf BA, Feroze Mahmood MD. Cardiac Imaging - 3-Dimensional Echocardiography. International Anesthesia Clinics. 2016;54 (1) :39-53.Abstract

Perioperative transesophageal echocardiography (TEE) has gradually become an essential intraoperative imaging modality, evolving from being used as a hemodynamic monitor to becoming an important procedural adjunct. Minimally invasive approaches for structural heart diseases have gained wider popularity due to advances in cardiac surgery, thereby making TEE imaging an integral component of intraoperative decision-making. Although traditional two-dimensional (2D) TEE provides a dynamic display of cardiac anatomy, it is spatially limited. Recent technological advances have allowed for the development of three-dimensional (3D) TEE. Currently, 3D imaging is mainly used for qualitative imaging of intra-cardiac structures and the data has been used for 3D printing of intra-cardiac structures. It has also demonstrated value in quantitative analyses of structural heart diseases and hemodynamic calculations. 3D imaging is gaining widespread popularity due to its useful applicability, allowing anesthesiologists to expand their role as perioperative physicians.

Matyal R, Montealegre-Gallegos M, Mitchell JD, H K, R B, KM H, D O'H, Wong V, Hess PE, Mahmood F. Manual Skill Acquisition During Transesophageal Echocardiography Simulator Training of Cardiology Fellows: A Kinematic Assessment. Journal of Cardiothoracic & Vascular Anesthesia. 2015;29 (6) :1504-10.Abstract


To investigate whether a transesophageal echocardiography (TEE) simulator with motion analysis can be used to impart proficiency in TEE in an integrated curriculum-based model.


A prospective cohort study.


A tertiary-care university hospital.


TEE-naïve cardiology fellows.


Participants underwent an 8-session multimodal TEE training program. Manual skills were assessed at the end of sessions 2 and 8 using motion analysis of the TEE simulator's probe. At the end of the course, participants performed an intraoperative TEE; their examinations were video captured, and a blinded investigator evaluated the total time and image transitions needed for each view. Results are reported as mean±standard deviation, or median (interquartile range) where appropriate.


Eleven fellows completed the knowledge and kinematic portions of the study. Five participants were excluded from the evaluation in the clinical setting because of interim exposure to TEE or having participated in a TEE rotation after the training course. An increase of 12.95% in post-test knowledge scores was observed. From the start to the end of the course, there was a significant reduction (p<0.001 for all) in the number of probe. During clinical performance evaluation, trainees were able to obtain all the required echocardiographic views unassisted but required a longer time and had more probe transitions when compared with an expert.


A curriculum-based approach to TEE training for cardiology fellows can be complemented with kinematic analyses to objectify acquisition of manual skills during simulator-based training.

Feng R, Rabya Saraf BA, Alex Shapeton MD, Robina Matyal MD, Roger Laham MD, Feroze Mahmood MD. A Complex Atrial Septal Defect and Three-Dimensional Echocardiography: A Question and an Answer. Journal of Cardiothoracic and Vascular Anesthesia. 2015.
Jelliffe Jeganathan MBBS, Ziyad Knio BS, Feroze Mahmood MD. Drawing Our Inferences with Transesophageal Echocardiography. Journal of Cardiothoracic and Vascular Anesthesia. 2015.